Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: LAG-3 and PD-1 levels were increased in DLBCL. (A) LAG-3 and PD-1 levels in DLBCL and normal lymph nodes. (B) Correlation between LAG-3 and PD-1 levels in DLBCL tissues. (C) PD-1 and LAG-3 levels in tissues detected by immunohistochemistry. (D) Linear correlation analysis of LAG-3 and PD-1 levels in DLBCL tissues.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques: Immunohistochemistry

Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: Prognosis analysis based on marker levels in DLBCL tissues. Kaplan-Meier analysis of patients with high and low PD-1 levels (A) , high and low LAG-3 levels (B) , high and low PD-1 and LAG-3 co-expression (C) , National Comprehensive Cancer Network-International Prognostic Index ≤ 3 and > 3 (D) , nodal and extranodal (E) , Eastern Cooperative Oncology Group score 0-1 and > 2 (F) , age > 60 and ≤ 60 years (G) . (H) Cox multivariate analysis of clinical prognosis of patients with DLBCL.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques: Marker, Expressing

Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: LAG-3 and PD-1 levels in diffuse large B-cell lymphoma were positively correlated with most infiltrating immune cells. (A) Differences in levels of 22 TILs in tumor and normal tissues. (B) Correlation of LAG-3 and PD-1 levels with those of 22 TILs in tumors. (C) Chord diagram of the results of correlation analysis between LAG-3 and TILs. (D) Chord diagram of the results of correlation analysis between PD-1 and TILs. Significance was defined as * P <0.05, *** P <0.001 and **** P <0.0001.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques:

Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: Expression of LAG-3 and PD-1 in CD4 + or CD8 + T cells in peripheral blood from the DLBCL and control groups. (A, B) Typical flow images of LAG-3 + CD4 + T and PD-1 + CD4 + T cells from patients with DLBCL and control groups and comparison plot. (C, D) Typical flow images of LAG-3 + CD8 + T and PD-1 + CD8 + T cells from patients with DLBCL and control groups and comparison plot. Significance was defined as * P <0.05, *** P <0.001. ns was defined as 'Not Significant'.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: Expression of LAG-3 and PD-1 on CD8 + T cells in peripheral blood from patients with DLBCL and healthy controls. Typical flow images of LAG-3 - PD-1 - CD8 + T cells (A) , LAG-3 + PD-1 - CD8 + T cells (B) , LAG-3 - PD-1 + CD8 + T cells (C) , and LAG-3 + PD-1 + CD8 + T cells (D) from patients with DLBCL and control groups and corresponding comparison plot. Significance was defined as *** p <0.001. ns, no significance.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Blockade of PD-1 and LAG-3 expression on CD8+ T cells promotes the tumoricidal effects of CD8+ T cells

doi: 10.3389/fimmu.2023.1265255

Figure Lengend Snippet: Flow cytometry images of LAG-3 and PD-1 on CD8 + T cells co-cultured with CD8 + T and SU-DHL6/OCI-LY3 cells. (A) Flow pattern of LAG-3 expression of CD8 + T cells after co-culture with CD8 + T and SU-DHL6 cells and corresponding comparison plot. (B) Flow patterns of PD-1 expression of CD8 + T cells co-cultured with SU-DHL6 after different treatments and corresponding comparison plot. (C) Flow patterns of LAG-3 expression of CD8 + T cells after co-culture with OCI-LY3 cells and different treatments and corresponding comparison plot. (D) Typical flow diagram of PD-1 expression on CD8 + T cells after co-culture with OCI-LY3 cells and corresponding comparison plot. Significance was defined as * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Tissue microarrays were immersed in citrate buffer and left to cool to room temperature, then treated with 3% hydrogen peroxide for 10 min, followed by addition of a drop goat serum after washing with PBS, and incubating at 37°C for 20 min. Next, samples were incubated with anti-PD-1 (Working fluid, Catalog Number: UMAB199, ZSGB-BIO) or anti-LAG-3 (1:2000, Catalog Number: 16616-1-AP, Proteintech) and chromogenic immunoassays performed with DAB after washing in PBS, followed by hematoxylin re-staining.

Techniques: Flow Cytometry, Cell Culture, Expressing, Co-Culture Assay